Journal: British Journal of Pharmacology

Article Title: Differences in the rate of oestrogen-induced apoptosis in breast cancer by oestradiol and the triphenylethylene bisphenol

doi: 10.1111/bph.12762

Figure Lengend Snippet: Effect of bisphenol on the growth and apoptosis of MCF7:5C breast cancer cells. (A) Cells were seeded in triplicate and treated with either control, E2 (1 nM) or bisphenol (BP, 1 μM), and the cells were harvested daily for 6 days. (B) Treatment with bisphenol versus control was extended for 13 days, and the DNA content of the remaining cells in each well was quantified. The data represent the mean of three independent experiments. (C) MCF7:5C cells were treated with control or bisphenol (1 μM) for 6 days and then stained with annexin V–FITC and propidium iodide and analysed by flow cytometry. Viable cells (left lower quadrant) are annexin V–FITC− and PI−; early apoptotic cells (right lower quadrant) are annexin V–FITC+ and PI−; dead cells (left upper quadrant) are PI+ and late apoptotic cells (right upper quadrant) are annexin V–FITC+ and PI+. An increased, late apoptotic effect is observed in the right upper quadrant.

Article Snippet: Real-time profiler assay RT-PCR profiler assay kits for apoptosis and cell cycle was used from a commercial vendor that uses 384-well plates (Cat# PAHS-3012E; Qiagen; SABiosciences Corp., Fredrick, MD, USA) to profile the expression of 370 apoptosis-related human genes and 4 × 96-well plates to profile the expression of 84 cell cycle-related genes (Cat# PAHS-020; Qiagen; SABiosciences Corp.).

Techniques: Control, Staining, Flow Cytometry

Journal: British Journal of Pharmacology

Article Title: Differences in the rate of oestrogen-induced apoptosis in breast cancer by oestradiol and the triphenylethylene bisphenol

doi: 10.1111/bph.12762

Figure Lengend Snippet: Determination of the trigger point for bisphenol-induced apoptosis. MCF7:5C cells were treated with bisphenol (BP, 1 μM) alone and 1 μM 4OHT was added and used to block and reverse bisphenol's action daily over a period of 9 days. The cells were harvested after 14 days of treatment. The DNA content of the remaining cells was quantified using a fluorescent DNA quantification kit. The point of trigger for apoptosis induced by bisphenol was determined as the time when the apoptotic effects of bisphenol could not be blocked by 4OHT.

Article Snippet: Real-time profiler assay RT-PCR profiler assay kits for apoptosis and cell cycle was used from a commercial vendor that uses 384-well plates (Cat# PAHS-3012E; Qiagen; SABiosciences Corp., Fredrick, MD, USA) to profile the expression of 370 apoptosis-related human genes and 4 × 96-well plates to profile the expression of 84 cell cycle-related genes (Cat# PAHS-020; Qiagen; SABiosciences Corp.).

Techniques: Blocking Assay

Journal: British Journal of Pharmacology

Article Title: Differences in the rate of oestrogen-induced apoptosis in breast cancer by oestradiol and the triphenylethylene bisphenol

doi: 10.1111/bph.12762

Figure Lengend Snippet: Caspase 4 is important for bisphenol (BP)-induced apoptosis. MCF7:5C cells were treated with control (0.1% ethanol) or bisphenol (1 μM) or caspase 4 (casp4) inhibitor with or without bisphenol for either (A) 12 days and assessed for cell proliferation or (B) for 6 days and evaluated for apoptosis. Apoptosis and inhibition of growth of cells were blocked by caspase 4 inhibitor z-LEVD-fmk (10 μM).

Article Snippet: Real-time profiler assay RT-PCR profiler assay kits for apoptosis and cell cycle was used from a commercial vendor that uses 384-well plates (Cat# PAHS-3012E; Qiagen; SABiosciences Corp., Fredrick, MD, USA) to profile the expression of 370 apoptosis-related human genes and 4 × 96-well plates to profile the expression of 84 cell cycle-related genes (Cat# PAHS-020; Qiagen; SABiosciences Corp.).

Techniques: Control, Inhibition